Canine Glucose Transporter 4 (GLUT4) ELISA Kit

Assay Genie's canine-specific immunoassay quantifies Glucose Transporter 4 (GLUT4) in samples collected from dogs, supporting veterinary and comparative metabolism research. GLUT4 is the insulin-regulated facilitative glucose transporter most strongly expressed in skeletal muscle, cardiac muscle, and adipose tissue, where its vesicle-to-membrane translocation drives insulin-stimulated glucose uptake. The kit supports studies of canine diabetes, obesity, and insulin resistance, including investigations of how exercise, diet, or candidate therapeutics alter transporter abundance in muscle and fat biopsies.

Each kit is configured for 96 individual determinations, allowing mid-throughput screening of cohorts or time-course experiments. Researchers can profile transporter expression in tissue homogenates prepared from freshly collected or archived canine biopsies, in cell lysates from cultured canine myotubes or adipocytes, and in fractions derived from subcellular fractionation protocols that separate plasma-membrane and intracellular-vesicle pools of GLUT4.

Assay Genie supplies the microplate, standards, detection antibodies, and substrate required to complete a full plate run. The photometric endpoint is read on a standard plate reader, and the workflow fits typical laboratory schedules for sample preparation, incubation, and signal development. Because the assay is calibrated and validated specifically for canine specimens, researchers comparing dog samples to other species should confirm cross-reactivity independently.