Canine Fibrinogen ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit uses a double-antibody format for the quantitative detection of fibrinogen in canine samples. It is intended for research applications and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, fecal extracts, and other liquid sample types.

The assay reports a sensitivity of 0.375 ng/mL and an assay range of 0.625–40 ng/mL. Reagents are stored at 2-8°C for 12 months, with light-sensitive components protected accordingly. The procedure involves sequential incubation steps, and absorbance is measured at 450 nm after addition of the stop solution.

Linearity was demonstrated for serum, EDTA plasma, and heparin plasma at dilution ratios of 1:2, 1:4, and 1:8. Recovery testing yielded average values of 95% in serum, 96% in EDTA plasma, and 96% in heparin plasma.