Canine Fas Ligand DIY ELISA Kit

The Canine Fas Ligand DIY ELISA Kit from Assay Genie enables researchers to quantify canine Fas Ligand (FasL/CD178), a TNF-family cytokine that triggers the extrinsic apoptosis pathway by binding the Fas receptor. The DIY format supplies uncoated plates and separate reagents so the user controls coating and workflow, with components sized for up to 10 plates per pack. Standard assay protocol uses 100 uL per well, with results read on a standard microplate reader after addition of stop solution. Reagents should be stored refrigerated at 4 degrees C, with the standard kept frozen at -20 degrees C in aliquots to limit freeze-thaw cycles; reconstituted material remains usable for the shelf life of the kit. The format supports immunology, transplant tolerance, and oncology research in canine models. Each pack includes pre-titrated capture and detection antibodies, recombinant canine FasL standard, streptavidin-HRP conjugate, TMB substrate, wash buffer, and protocol documentation intended for research use only.