Canine Complement C3/C3 ELISA Kit

Designed by Assay Genie for canine immunology research, this Sandwich ELISA kit enables quantitative measurement of complement C3 in canine samples. The double-antibody sandwich format uses a biotin-labelled detection antibody and HRP-streptavidin (SABC) with TMB substrate for colorimetric detection, read at 450 nm, supporting studies of complement activation, inflammatory responses, and conditions such as autoimmune disease and infection in dogs.

The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported sensitivity is 9.375 pg/mL with a working range of 15.625–1000 pg/mL. Dilutional linearity across 1:2, 1:4, and 1:8 samples is 82–98% for serum, 80–100% for EDTA plasma, and 82–100% for heparin plasma; spike-and-recovery averages are 96% for serum, 95% for EDTA plasma, and 96% for heparin plasma. Kit components are stored at 2–8°C and remain stable for 12 months.

The workflow runs through reagent equilibration, primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB colour development in the dark for 10–20 minutes, and a stop reaction measured immediately at 450 nm.