Canine Coagulation Factor VIII (F8) ELISA Kit

Veterinary coagulation research relies on accurate measurement of Factor VIII (F8), the plasma glycoprotein cofactor whose functional deficiency underlies canine Hemophilia A. This immunoassay from Assay Genie provides a quantitative tool for evaluating F8 antigen levels in dog plasma, supporting studies of inherited bleeding disorders, hepatic protein synthesis, and acquired coagulopathies linked to immune-mediated disease or disseminated intravascular coagulation.

Plasma collected into citrate anticoagulant is added directly to the pre-coated microplate wells, after which a labelled detection antibody is introduced to complete the capture format that binds F8 at the plate surface. Following substrate development, absorbance readings are compared against a standard curve generated from the calibrators supplied with the kit. Each kit contains reagents sufficient for 96 determinations, with all buffers, antibodies, and standards pre-prepared and ready to use. The protocol is intended for research use only, supporting bench workflows that pair standard laboratory incubation and washing equipment with plate-based absorbance readout.