Canine ADORA1/Adenosine receptor A1 ELISA Kit

A Sandwich ELISA, Double Antibody-based immunoassay from Assay Genie designed for the precise measurement of Adora1/Adenosine receptor A1 levels in canine samples. The kit uses a colorimetric detection method and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Storage is recommended at 2–8°C for 12 months.

The Adora1 adenosine receptor A1 mediates the effects of adenosine, a key signaling molecule involved in various physiological processes, and its dysregulation has been linked to cardiovascular, neurological, and inflammatory conditions. The kit is intended for studying the receptor's role in health and disease and for developing potential therapeutic interventions.

The assay procedure includes equilibration of reagents and TMB substrate to room temperature, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, color development with TMB substrate in the dark for 10–20 minutes, and reading absorbance at 450 nm immediately after adding stop solution.