CAMK2A/CAMK2D Colorimetric Cell-Based ELISA Kit

This cell-based ELISA kit from Assay Genie is designed for the qualitative measurement of CAMK2A and CAMK2D protein expression directly in cultured cells. CAMK2A and CAMK2D are members of the calcium/calmodulin-dependent protein kinase family, playing key roles in intracellular signaling pathways and regulating various cellular functions. Dysregulation of these proteins has been linked to diseases including cancer, neurological disorders, and cardiovascular diseases.

The assay uses a colorimetric detection method read at 450 nm and is reactive with human, mouse, and rat samples. It is suitable for adherent cells and has a stated dynamic range of > 5000 Cells. The kit is supplied in a 96-Well Microplate format with storage stability at 4°C for 6 months.

Detection follows an indirect ELISA format in which target proteins are captured by specific primary antibodies and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, while Crystal Violet whole-cell staining can be used to determine cell density and adjust for plating differences. The lysate-free, high-throughput workflow supports screening of treatments, inhibitors (such as siRNA or chemicals), and activators on CAMK2A/CAMK2D expression.