c-Jun (Phospho-Thr93)Colorimetric Cell-Based ELISA Kit

The Assay Genie c-Jun (Phospho-Thr93) Colorimetric Cell-Based ELISA Kit is a cell-based ELISA designed for the qualitative detection of phosphorylated c-Jun at threonine 93 in cells. The kit is reactive with human, mouse, and rat samples and uses a colorimetric detection method measured at 450 nm, with a stated dynamic range of > 5000 Cells.

This lysate-free, high-throughput assay kit can detect c-Jun protein phosphorylation and expression profiles in cultured cells and is suitable for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on c-Jun phosphorylation. The assay uses an indirect ELISA format: c-Jun (Phospho-Thr93) is captured by phospho-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining can be used to determine cell density.

Phosphorylation of c-Jun at threonine 93 is a post-translational modification that regulates its activity as a transcription factor involved in cell proliferation, survival, and differentiation. Abnormal c-Jun phosphorylation has been implicated in cancer, inflammatory disorders, and neurodegenerative diseases, making it a relevant biomarker for research and drug development studies focused on intracellular signalling pathways.