c-Jun (Phospho-Thr239)Transcription Factor Activity Assay

This DNA-binding ELISA kit from Assay Genie enables detection and qualitative analysis of c-Jun (Phospho-Thr239) active transcription factors in eukaryotic nuclear or cell lysates. It uses a colorimetric detection method, and reported reactivity includes human, mouse and rat. c-Jun, also known as Activator protein 1 (AP1), is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3', helping to regulate gene expression.

Phosphorylation of c-Jun by HIPK3 promotes activity of NR5A1, leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation; c-Jun is also involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer cells. Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.