c-Jun (Phospho-Ser63)Colorimetric Cell-Based ELISA Kit

A cell-based ELISA kit from Assay Genie designed for the qualitative detection of phosphorylated c-Jun at serine 63 in cultured cells. The kit uses an indirect ELISA format, in which target-specific primary antibodies capture c-Jun (Phospho-Ser63) and HRP-conjugated secondary antibodies enable a colorimetric readout at 450 nm. Reactivity covers human, mouse, and rat samples, and the assay is suitable for adherent cells as well as screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on c-Jun phosphorylation.

Phosphorylation of c-Jun at serine 63 is a critical event in the activation of this transcription factor, which regulates cellular processes including proliferation, differentiation, and apoptosis, and aberrant phosphorylation has been implicated in cancer, inflammatory disorders, and neurodegenerative diseases. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining is used to determine cell density. The dynamic range is > 5000 Cells, and the product falls within the Epigenetics and Nuclear Signaling research area.

Associated gene information: Gene Symbol JUN, Gene ID 3725, UniProt ID P05412, OMIM 165160, Unigene Hs.714792. c-Jun (also known as AP-1 or transcription factor AP-1) recognizes the enhancer heptamer motif 5'-TGA[CG]TCA-3'.