C/EBP-beta (Phospho-Thr235/188)Colorimetric Cell-Based ELISA Kit

A Cell-Based ELISA from Assay Genie for the detection of C/EBP-beta phosphorylated at Thr235/188 in cultured cells. The kit uses a colorimetric readout at 450 nm in an indirect ELISA format, where target-specific primary antibodies capture the phosphorylated protein and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition. It is described as a convenient, lysate-free, high-throughput and sensitive assay for measuring relative phosphorylation levels in cells.

Reactivity is reported for human, mouse and rat, with a stated dynamic range of > 5000 Cells. Because the assay is qualitative, two normalization methods are supplied: a monoclonal anti-GAPDH antibody serves as an internal positive control for the target signal, and Crystal Violet whole-cell staining is provided to determine cell density so absorbance values can be normalized to cell amounts and plating differences adjusted. The kit can be used to screen the effects of various treatments, inhibitors (such as siRNA or chemicals) or activators on C/EBP-beta phosphorylation.

C/EBP-beta (gene symbol CEBPB; UniProt P17676; Gene ID 1051) is a basic leucine zipper transcription factor that regulates genes involved in immune and inflammatory responses, adipogenesis, gluconeogenesis, liver regeneration, and hematopoiesis. Phosphorylation at Thr235/188 has been linked to its activation and to roles in cellular differentiation, proliferation, and inflammation, with research relevance to cancer, metabolic disorders, and inflammatory conditions. The sub-type is Phospho, the sample type is adherent cells, and the listed research area is Developmental Biology.