C/EBP-beta Colorimetric Cell-Based ELISA Kit

From Assay Genie, this C/EBP-beta Colorimetric Cell-Based ELISA Kit is a cell-based assay designed for the accurate detection of C/EBP-beta levels in samples such as cell lysates, supernatants, serum, and plasma. It uses a colorimetric detection method read at 450 nm and is supplied in a 96-Well Microplate format. The kit is reactive with Human, Mouse, and Rat samples and is positioned for research in the developmental biology area. C/EBP-beta (gene symbol CEBPB, Gene ID 1051, UniProt ID P17676) is a transcription factor involved in immune and inflammatory responses, adipogenesis, liver regeneration, and hematopoiesis.

The assay is described as lysate-free and high throughput, providing a qualitative measure of relative C/EBP-beta levels through an indirect ELISA format. C/EBP-beta is captured by specific primary antibodies, and HRP-conjugated secondary antibodies catalyse a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density after the colorimetric read. The kit has a dynamic range of greater than 5000 cells, should be stored at 4 °C, and carries a stated stability of 6 months. Researchers can use it to measure the relative amounts of C/EBP-beta in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on C/EBP-beta expression.