C/EBP-alpha (Phospho-Thr226)Colorimetric Cell-Based ELISA Kit

Designed for the accurate detection of phosphorylated CCAAT/enhancer-binding protein alpha (C/EBP-alpha) at threonine 226 in cell lysates, this colorimetric cell-based ELISA kit from Assay Genie offers high sensitivity and specificity for researchers studying the role of C/EBP-alpha phosphorylation in cellular processes. C/EBP-alpha is a critical transcription factor that regulates gene expression involved in cell differentiation, proliferation, and metabolism, and phosphorylation at threonine 226 has been linked to various diseases, including cancer, diabetes, and liver disorders.

The assay uses an indirect ELISA format in which the target is captured by phospho-specific primary antibodies, with HRP-conjugated secondary antibodies catalysing a colorimetric reaction upon substrate addition. A monoclonal GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density. The kit is reactive with human, mouse and rat samples, is intended for use with adherent cells, and performs detection colorimetrically at 450 nm with a dynamic range of > 5000 cells.

C/EBP-alpha is a bZIP transcription factor that can form homodimers or heterodimers with CEBP-beta and CEBP-gamma, binds the promoter and modulates expression of the gene encoding leptin, and can interact with CDK2 and CDK4, inhibiting these kinases and causing growth arrest in cultured cells. The gene symbol is CEBPA, with Gene ID 1050 and UniProt ID P49715, and mutation of this gene is associated with acute myeloid leukemia.