C/EBP-alpha (Phospho-Ser21)Colorimetric Cell-Based ELISA Kit

A cell-based ELISA from Assay Genie designed for the precise measurement of C/EBP-alpha (Phospho-Ser21) levels in cell lysates. C/EBP-alpha is a bZIP transcription factor that regulates gene expression and cell differentiation, and phosphorylation at Ser21 modulates its activity, making it a relevant target for research in areas such as cancer, inflammation, and metabolism. The kit detects C/EBP-alpha protein phosphorylation and expression profiles in cultured cells and can be used to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on C/EBP-alpha phosphorylation.

The assay uses a colorimetric detection method at 450 nm in an indirect ELISA format. Target-specific primary antibodies capture C/EBP-alpha (Phospho-Ser21), and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. The kit is reactive with Human, Mouse, and Rat samples, has a stated dynamic range of > 5000 cells, and is intended for use with adherent cells.

Because the assay is qualitative, two normalisation approaches are provided: a monoclonal antibody specific for human GAPDH serves as an internal positive control, and Crystal Violet whole-cell staining can be used to determine cell density, allowing absorbance values to be normalised to cell number and plating differences to be adjusted. The kit offers a convenient, lysate-free, high-throughput format.