BTK Colorimetric Cell-Based ELISA Kit

Designed for the precise measurement of Bruton's tyrosine kinase (BTK) activity in cell lysates, this cell-based ELISA kit from Assay Genie offers a lysate-free, high-throughput, and sensitive workflow for profiling BTK protein expression in cultured cells. The kit supports screening for the effects of various treatments, inhibitors (such as siRNA or chemicals), and activators on BTK, with qualitative determination achieved through an indirect ELISA format in which BTK-specific primary antibodies are bound by HRP-conjugated secondary antibodies that catalyse a colourimetric reaction upon substrate addition.

A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, while a Crystal Violet whole-cell staining method can be used to determine cell density and adjust for plating differences. The assay shows reactivity with human, mouse, and rat samples and is intended for use with adherent cells.

Specifications include a colourimetric detection method read at 450 nm, a dynamic range of > 5000 cells, storage at 4°C for 6 months, and a 96-well microplate format. The kit is classified for immunology research and targets BTK, a key regulator of B cell development and function whose dysregulation has been implicated in conditions such as leukemia, lymphoma, and rheumatoid arthritis.