Bovine Vasoactive Intestinal Peptide (VIP) ELISA Kit

This competitive immunoassay from Assay Genie is engineered for quantitative measurement of VIP, a 28-residue neuropeptide implicated in smooth muscle relaxation, vasodilation, and enteric neurotransmission. In bovine models, VIP is studied for its role in gastrointestinal motility regulation, exocrine pancreatic function, reproductive tract signaling, and pulmonary vascular tone, where it acts through VPAC1 and VPAC2 G protein coupled receptors.

The kit is supplied as a microplate format with all reagents needed to run a complete competitive curve. Unopened kits should be stored refrigerated and protected from light; reconstituted standards and the biotinylated VIP conjugate remain stable when aliquoted and held frozen at negative 20 degrees C, avoiding repeated freeze-thaw cycles to preserve peptide integrity.

Validated for work with bovine-origin matrices across multiple sample types, the assay supports both in vitro cell-based experiments and in vivo animal research applications, including studies of inflammatory bowel disease mechanisms, neuroimmune crosstalk, and reproductive biology. Intended for research use only, each package contains the precoated antibody plate, lyophilized VIP standards, detection reagents, and substrate solution required for ninety-six individual determinations.