Bovine Tumor necrosis factor (TNF) ELISA Kit

Assay Genie provides a sandwich ELISA configured for the quantitative measurement of bovine tumor necrosis factor (TNF) using a colorimetric detection method. The kit is validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and shows specificity for natural and recombinant bovine TNF. Listed synonyms for the target include Cachectin, TNF-alpha, tumor necrosis factor ligand superfamily member 2, TNF-a, TNFA and TNFSF2.

Performance specifications cited in the product documentation include a detection range of 0.156-10ng/mL and a sensitivity of 0.067ng/mL. Kit components are stored at -20°C or 4°C depending on the reagent, and the product is designated for research use only. The target is described as a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR, is mainly secreted by macrophages, and contributes to inflammation, immune regulation, cell survival and tumour cell death; the intracellular domain form induces IL12 production in dendritic cells. The associated UniProt record (Q06599, NCBI Gene ID 280943) lists the protein as a homotrimer with a molecular weight of 25,439 Da, and the research area is given as Neurosciences.