Bovine Transcriptional activator Myb (MYB) ELISA Kit

The Assay Genie sandwich ELISA kit targets the bovine MYB protein, a transcriptional activator implicated in hematopoietic cell proliferation, differentiation, and apoptosis regulation. The microplate format uses colorimetric detection at 450 nm with sensitivities in the pg/ml range, allowing quantitative measurement of MYB concentrations in serum and plasma samples. Each kit contains a pre-coated 96-well plate, calibrated standards, biotinylated detection antibodies, wash buffer, substrate solution, and stop reagent sufficient for 96 determinations. Recommended storage is at 2-8 °C with all components stable until the printed expiration date; reagents should not be subjected to repeated freeze-thaw cycles. The assay is intended for research use only and is validated for bovine samples, supporting studies in veterinary immunology, leukemia research, and gene expression investigations involving the MYB transcription factor family.

The Assay Genie MYB ELISA Kit targets the c-Myb proto-oncogene product, a 75-80 kDa nuclear transcription factor central to definitive hematopoiesis. In bovine models, MYB expression is concentrated in immature myeloid and lymphoid progenitors of the bone marrow and thymus, with declining levels as cells mature, supporting research on stem and progenitor cell populations. The sandwich ELISA uses a pair of monoclonal antibodies against distinct epitopes of full-length MYB, providing specificity over the related family members A-Myb and B-Myb. The standard curve spans 15-1000 pg/ml with intra-assay CV under 10% and inter-assay CV under 12%. Bring all reagents to room temperature before use, prepare the wash buffer per protocol, and prevent the microplate from drying between incubations. The 96-well format permits flexible plate layouts for batched serum and plasma testing alongside standards and internal controls. The kit complements downstream methods such as qPCR, western blot, and flow cytometry in studies of bovine leukocyte biology, vaccine response profiling, and investigation of MYB-regulated oncogenic pathways in veterinary oncology research use only applications.