Bovine TL-3 (Interleukin-3) ELISA Kit (BOFI00131)

Bovine Interleukin-3 (IL-3) is a pleiotropic cytokine that drives proliferation and maturation of multilineage hematopoietic progenitors, making its quantification relevant to studies of innate immunity, mucosal defense, and vaccine responses in cattle. This Assay Genie kit supports research workflows that correlate circulating IL-3 levels with mastitis susceptibility, parasitic infection outcomes, and stress-induced leukocyte shifts in dairy and beef herds. The microplate is pre-coated with a bovine-reactive capture antibody, paired with a biotinylated detection antibody and streptavidin-HRP conjugate for chromogenic readout. Each box contains one strip plate, lyophilized recombinant bovine IL-3 standards, assay diluent, wash concentrate, TMB substrate, and stop solution, sufficient for 96 individual determinations. A typical detection range of 15.6 to 1000 pg/mL and sensitivity near 7.8 pg/mL allows measurement across physiological and stimulated cytokine concentrations, while a 50 uL sample volume per well fits routine clinical specimens from cattle. Reagents ship refrigerated and should remain refrigerated until first use; avoid repeated freeze-thaw cycles of reconstituted standards and bring all buffers to room temperature before running the assay.