Bovine Syndecan-2 (SDC2) ELISA Kit

Syndecan-2 (SDC2) is a transmembrane heparan sulfate proteoglycan involved in cell-matrix adhesion, growth factor signaling, and cytoskeletal organization. This Sandwich ELISA from Assay Genie enables quantitative measurement of bovine SDC2 in serum and plasma using a colorimetric detection method on a 96-well microplate format. Each kit provides all reagents necessary for running standard curves and unknown samples, with results typically read at 450 nm on a standard microplate reader. The assay is intended for research use only and supports in vitro studies of bovine vascular biology, cartilage development, and comparative models of epithelial homeostasis. Reagents should be stored refrigerated at 2-8 °C unless otherwise indicated, with the standard protected from light. The complete kit format supports consistent inter-assay reproducibility across the full 96-well plate when standard curves are run in duplicate.

As a transmembrane heparan sulfate proteoglycan also known as fibroglycan, bovine SDC2 anchors three HS chains that bind FGF2, VEGF, and Wnt ligands, making quantitative measurement useful for studies of angiogenesis and matrix remodeling. The Assay Genie sandwich ELISA captures the bovine core protein between an immobilized capture antibody and a biotinylated detection antibody, with streptavidin-HRP and TMB substrate producing the colorimetric signal. Standard curves typically span 0.31-20 ng/mL, with intra-assay CV under 8% and inter-assay CV under 10%. Reconstituted standards should be aliquoted and stored at -20 °C to limit freeze-thaw cycles, while working dilutions are kept refrigerated at 2-8 °C and used within 24 hours. The kit includes a pre-coated 96-well plate, lyophilized recombinant bovine SDC2 standard, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer concentrate, and sample diluent. Total assay time is roughly 3.5 hours from sample addition to final stop, and absorbance is read at 450 nm with 570 nm background correction. Plasma collected in EDTA or citrate tubes is preferred over heparin, which can interfere with the heparan sulfate recognition epitope. Researchers studying bovine cartilage, vascular smooth muscle proliferation, and epithelial barrier models commonly pair this kit with SDC1 and SDC4 ELISAs for parallel proteoglycan profiling.