Bovine Sodium/calcium exchanger 1 (SLC8A1) ELISA Kit

The Assay Genie Bovine SLC8A1 Sandwich ELISA Kit quantifies the sodium/calcium exchanger 1 (NCX1), a key membrane antiporter most abundant in cardiac myocytes that exchanges three Na+ ions for one Ca2+ ion to regulate calcium homeostasis. This colorimetric immunoassay is calibrated for serum and plasma samples, making it applicable for cardiovascular research, ion transport studies, and comparative bovine physiology. Each kit ships as a complete 96-well microplate with pre-coated capture antibody, biotinylated detection reagent, streptavidin-HRP conjugate, and a ready-to-use standard curve, typically accommodating 40 samples run in duplicate. The protocol involves a brief room-temperature incubation step followed by refrigerated storage of working solutions and is intended for research use only. For best stability, store kit components at 2-8°C, avoid repeated freeze-thaw cycles, and protect reagents from light. A standard curve spanning the expected analyte concentration range allows researchers to interpolate unknown sample values, with sensitivity suitable for quantifying low abundance NCX1 protein in plasma and serum matrices.

The Assay Genie SLC8A1 (NCX1) Sandwich ELISA Kit is calibrated against recombinant bovine antigen and uses a monoclonal capture antibody paired with a biotin-streptavidin-HRP detection cascade. TMB substrate produces a measurable signal at 450 nm on a standard microplate reader. Each 96-well plate contains pre-determined strip wells removable for partial-plate runs, and the included standard is reconstituted in sample diluent to generate a seven-point series, typically spanning 0.156–10 ng/mL, allowing interpolation of unknown serum or plasma values. The recommended protocol loads 100 µL of sample or standard per well, incubates 90 minutes at 37 °C, performs four wash cycles, and adds 50 µL each of detection antibody and streptavidin-HRP, with a final 15–20 minute substrate step terminated by 50 µL of stop solution (2 M sulfuric acid). Reported intra-assay precision is typically below 10% CV and inter-assay CV below 15% across independent lots, with no appreciable cross-reactivity observed against NCX2 (SLC8A2) or NCX3 (SLC8A3) under standard conditions. Researchers in cardiovascular physiology, ischemia-reperfusion injury modeling, and comparative veterinary cardiology use this kit to track NCX1 expression changes following pharmacologic intervention, transgenic manipulation, or dietary electrolyte modulation. All components arrive liquid-ready or lyophilized in individually sealed vials, including 20× wash buffer concentrate, assay diluent, TMB, stop reagent, and adhesive plate sealer, with a printed protocol card inserted in the kit box. Unopened kits are stable at 2–8 °C for up to 6 months; reconstituted standards should be aliquoted and stored frozen at –20 °C to limit repeated freeze-thaw cycles beyond three rounds.