Bovine serum albumin (BSA) ELISA Kit (BOFI00128)

The Assay Genie competitive immunoassay enables quantitative measurement of bovine serum albumin across experimental workflows requiring tight contamination control. Researchers commonly deploy this kit to verify residual BSA levels in recombinant protein preparations, monitor cross-contamination in cell culture media, and validate purification steps in downstream bioprocessing. Each kit supplies pre-coated microplate wells, calibration standards, and detection reagents sufficient for the full plate run. Standard curves are generated from serial dilutions of the provided reference antigen, allowing interpolation of unknown sample concentrations. Sensitivity is reported at approximately 0.1 ug/mL with a working detection range of 0.156 to 10 ug/mL, supporting both trace-level and mid-range quantitation. The recommended sample volume is 50 uL per well, applied in duplicate to maximize reproducibility. Reagent components should be stored refrigerated at 2 to 8 degrees C and protected from light; freeze-thaw cycles of the conjugate should be avoided to preserve activity. The assay is intended for research use only and is not validated for diagnostic or clinical applications.