Bovine Selectin, Endothelium (SELE) ELISA Kit

The Assay Genie bovine SELE ELISA kit delivers quantitative measurement of E-selectin, the endothelial-leukocyte adhesion molecule transiently expressed on activated vascular endothelium. Researchers use this 96-assay format to study inflammatory cascades, endothelial activation, and neutrophil trafficking in cattle, with relevance for investigations of mastitis, endotoxemia, and bovine reproductive disorders.

Compatible sample matrices span serum samples, plasma, tissue homogenates, cell lysates, and cell culture supernatants, giving flexibility for both in vivo and in vitro experimental designs. The assay relies on colorimetric signal development read on a standard microplate reader, with all reagents—pre-coated wells, detection antibody, standards, and substrate—supplied in a single kit box.

For reliable performance, store unopened kits refrigerated and allow components to equilibrate to room temperature before running the protocol. Avoid freeze-thaw cycles of the conjugate and standard beyond what the protocol specifies. Researchers should consult the lot-specific product insert for exact standard curve concentrations, recommended dilution factors, and validated sample volumes tailored to each matrix type.