Bovine Prothrombin (F2) ELISA Kit

This Sandwich ELISA from Assay Genie is designed for the detection of bovine Prothrombin (F2), also known as coagulation factor II. The assay is reactive with bovine samples and is intended for research use only.

The kit is validated for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and shows specificity for natural and recombinant bovine Prothrombin. Listed performance characteristics include a detection range of 7.8-500ng/mL and a sensitivity of 3.7ng/mL; intra-assay CV, inter-assay CV, linearity, and recovery values are provided with the kit.

Prothrombin (UniProt P00735, gene symbol F2, NCBI Gene ID 280685) is described as a secreted, extracellular-space protein with a listed molecular weight of 72 kDa. It is reported as a heterodimer (alpha-thrombin) of disulfide-linked light and heavy chains and is reported to form a heterodimer with SERPINA5. According to the listed UniProt function, thrombin cleaves bonds after Arg and Lys, converts fibrinogen to fibrin, and activates factors V, VII, VIII, XIII and, in complex with thrombomodulin, protein C, contributing to blood homeostasis, inflammation and wound healing. The listed research area is cancer.