Bovine Protachykinin-1 (TAC1) ELISA Kit

The Bovine Protachykinin-1 (TAC1) ELISA Kit from Assay Genie employs a quantitative sandwich immunoassay configured on a 96-well microplate. The TAC1 gene encodes the precursor protachykinin-A polypeptide, which is proteolytically processed to release bioactive tachykinin neuropeptides including Substance P and Neurokinin A, mediators implicated in nociception, neurogenic inflammation, and smooth muscle contraction. Researchers measuring circulating tachykinin precursor levels in cattle can apply serum or plasma samples directly to pre-coated wells, where immobilized capture antibodies sequester the target peptide. A biotinylated detection antibody and streptavidin-HRP conjugate generate a colorimetric signal proportional to analyte concentration, with absorbance read at 450 nm following TMB substrate addition.

Each kit contains a precoated 96-well plate, lyophilized standards, detection antibody concentrate, streptavidin-HRP, wash buffer, substrate solution, and stop reagent sufficient for 96 individual determinations. Standard curves are typically constructed across a working range calibrated in pg/mL, with intra- and inter-assay precision documented in the enclosed certificate of analysis. Unopened kits should be stored at 2-8°C; reconstituted reagents and prepared standard curves are best aliquoted and held frozen to limit freeze-thaw cycles. The assay is intended for research use only and supports investigations into bovine reproductive biology, stress physiology, gastrointestinal motility, and inflammatory disease models.

From a practical bench standpoint, Assay Genie's bovine TAC1 sandwich ELISA tolerates serum and plasma matrices commonly harvested from cattle in reproductive, metabolic, and infectious-disease studies. Typical runs load 100 µL of sample per well against a lyophilized standard curve bracketed in pg/mL, with detection limits often falling in the low picogram range when undiluted neat samples are analyzed. The 96-well microplate layout supports duplicate or triplicate standards alongside unknowns, and the streptavidin-HRP / TMB chromogenic step produces a stable color read at 450 nm that can be corrected using a 570 nm or 630 nm reference wavelength if plate imperfections are a concern.

Investigators working on stress-axis physiology frequently pair TAC1 measurements with cortisol or ACTH assays from the same serum draw, since Substance P and Neurokinin A are co-released during nociceptive and inflammatory challenges. In dairy and beef production research, the kit is applied to track tachykinin dynamics around parturition, mastitis episodes, and gastrointestinal parasite burdens. Reconstituted standards and biotinylated antibody concentrate should be aliquoted into single-use vials to limit freeze-thaw cycles, while unused strip wells remain stable at 2-8°C in the resealed foil pouch with desiccant until the kit expiry printed on the outer label.