Bovine Pituitary adenylate cyclase-activating polypeptide (ADCYAP1) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the accurate quantitative detection of bovine Pituitary adenylate cyclase-activating polypeptide (ADCYAP1), also referred to by the synonym PACAP. The assay is validated for use with bovine samples and accepts serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with stated specificity for both natural and recombinant bovine ADCYAP1.

According to the manufacturer's specification, the kit provides a detection range of 15.6-1000pg/ml and a sensitivity of 5.9pg/ml. The assay is performed as a sandwich-format ELISA with the signal read on a standard microplate reader at 450 nm. The target corresponds to UniProt accession Q29W19, with a reported molecular weight of 19,464 Da (NCBI Gene ID: 615187). ADCYAP1/PACAP is described as a secreted neuropeptide implicated in neurotransmission, neuroprotection, hormone regulation, cell proliferation, and stress responses; it signals through receptor-mediated G protein activation and adenylate cyclase stimulation, and contributes to glucose homeostasis and insulin secretion by pancreatic beta cells. The kit is supplied in a solid-phase format intended for storage at -20°C and is labelled for research use only.