Bovine Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (PPCK1) ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA kit is designed for the precise quantification of cytosolic phosphoenolpyruvate carboxykinase [GTP] (PCK1) in bovine samples. The assay is reactive against bovine analyte and shows specificity for natural and recombinant bovine PCK1, also referenced under the synonyms PEPCK-C and PPCK1.

Validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, the assay delivers a detection range of 0.156-10 ng/ml with a sensitivity of 0.065 ng/ml. The target is a monomeric cytoplasmic enzyme (UniProt Q8HYZ4, molecular weight 69,353 Da) that catalyses the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Abnormal levels of cytosolic GTP have been associated with metabolic disorders and insulin resistance, making PCK1 a valuable biomarker within the cardiovascular research area. For research use only.