Bovine Pg (Progesterone) ELISA Kit

Assay Genie's competitive ELISA is designed for the precise measurement of progesterone (Pg) in bovine samples. The colorimetric, antigen-coated assay supports reproductive research in cattle, including estrus detection, pregnancy confirmation, and management of reproductive disorders, by providing accurate and reproducible quantification of this key reproductive hormone.

The kit accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Performance figures stated by the manufacturer include a sensitivity of 0.188 ng/mL and an assay range of 0.313–20 ng/mL. Spiked recovery in serum, EDTA plasma, and heparin plasma averages between 91% and 95%. The workflow involves a biotin-labeled antibody binding step at 37 °C, TMB-based color development read at 450 nm, and a sealed storage condition of 2–8 °C with no cryopreservation. Provided components include a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody concentrate, HRP-streptavidin conjugate, TMB substrate, dilution and wash buffers, stop solution, plate sealers, and a technical manual.