Bovine Peroxisome Proliferator Activated Receptor Gamma (PPARg) ELISA Kit

The Assay Genie Bovine PPARg ELISA Kit supports quantitative measurement of peroxisome proliferator-activated receptor gamma across multiple bovine research matrices, including plasma fractions, serum samples, tissue homogenates, cell lysates, and cell culture supernatants. Each kit contains a pre-coated microplate with removable strip wells, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated reference standards, and TMB substrate sufficient for 96 individual determinations, with a recommended sample volume of 100 µL per well.

PPARg is a nuclear receptor governing adipocyte differentiation, lipid metabolism, glucose homeostasis, and inflammatory gene expression, making this reagent relevant to metabolic disorder research, livestock physiology studies, and obesity investigations in cattle models. The assay is calibrated specifically for the bovine PPARg isoform and is not intended to cross-react with human or murine homologs. The procedure follows a multi-step antibody-based workflow with absorbance measurement after stopping the enzymatic color development reaction.

Intended for research use only and not for diagnostic or therapeutic procedures. Reagents should be stored at the temperature indicated on the vial labels, with reference standards protected from light and subjected to minimal freeze-thaw cycles to preserve binding activity.