Bovine Ornithine Carbamoyl Transferase (OCT) ELISA Kit

Ornithine transcarbamylase activity in cattle serves as a sensitive biomarker of hepatocellular damage, and this Assay Genie immunoassay kit provides a quantitative plate-based workflow for measuring the enzyme in bovine research samples.

OCT catalyzes the condensation of ornithine with carbamoyl phosphate to form citrulline within the hepatic urea cycle, and elevated circulating levels follow hepatocyte membrane disruption from toxic, nutritional, or inflammatory insults. The kit is configured as a 96 assays format and ships with a pre-coated microplate, lyophilized calibrators, detection antibody, assay diluent, concentrated wash buffer, TMB substrate, and stop solution, with a protocol sufficient to generate a standard curve and interpolate unknown concentrations.

Typical research applications include monitoring liver damage following mycotoxin exposure, evaluating the hepatoprotective effects of feed additives or rumen-protected nutrients, profiling hepatic responses to drug candidates, and characterizing inherited or acquired urea cycle disturbances in bovine models. The kit is intended for laboratory research use only and is not validated for veterinary diagnostic application in production herds.