Bovine Neuropeptide Y (NPY) ELISA Kit

Neuropeptide Y (NPY) is a 36-amino acid peptide involved in appetite regulation, stress response, and cardiovascular function. This competitive immunoassay from Assay Genie enables quantitative measurement of NPY in bovine-derived samples using a 96-assay microplate format. The kit supports research applications in veterinary neuroscience, metabolic disease models, and reproductive biology studies where cattle serve as translational models.

Each kit contains pre-coated plates and reagents validated for use with tissue homogenates, cell lysates, and cell culture supernatants. Researchers investigating feed intake regulation, hypothalamic signaling, or bovine growth physiology will find this assay useful for generating reproducible concentration data across experimental cohorts.

The procedure relies on competitive binding, where sample NPY competes with conjugated NPY for antibody binding sites. After incubation and wash steps, a colorimetric readout is produced and quantified against a standard curve generated from calibrated NPY references. The complete workflow fits within standard laboratory timelines and requires only standard plate reader equipment.

Storage temperature requirements follow standard immunoassay handling, with the kit shelf life supporting extended longitudinal studies. Each lot is quality-controlled to ensure consistent performance across runs, supporting multi-site collaborations. The 96-assay microplate format provides flexibility for both small pilot experiments and larger screening campaigns, with sufficient wells for standards, controls, and samples in a single run.