Bovine Matrix Metalloproteinase 3 (MMP3) ELISA Kit

Quantitative measurement of stromelysin-1 (MMP3) in cattle-derived research samples is supported by the Assay Genie Bovine MMP3 ELISA Kit, a microplate-based immunoassay configured for in vitro use. MMP3 is a zinc-dependent endopeptidase secreted by chondrocytes, synoviocytes, and stromal cells that cleaves extracellular matrix substrates including fibronectin, laminin, cartilage proteoglycans, and type IV collagen. The protein is a relevant biomarker in studies of bovine osteoarthritis, mastitis-associated tissue remodeling, and reproductive tract changes through the estrous cycle.

The kit uses matched antibody pairs immobilized on a microplate to capture the target from diluted samples, with detection completed by an enzyme-linked secondary antibody and a chromogenic substrate read on a standard plate reader. Reagents should be stored refrigerated between uses, and all components are intended for research use only, not for diagnostic procedures.

Each package includes the coated microplate, reference standards, detection antibody, enzyme conjugate, wash concentrate, substrate solution, stop reagent, plate sealers, and an assay protocol booklet. Bovine veterinary researchers, dairy scientists, and reproductive biologists apply this assay to monitor MMP3 concentrations in experimentally induced or naturally occurring disease states.