Bovine Matrix Metalloproteinase 1 (MMP1) ELISA Kit

The Assay Genie bovine MMP1 ELISA kit targets interstitial collagenase, the enzyme responsible for initiating cleavage of fibrillar type I, II, and III collagens in connective tissues. Researchers use quantitative MMP1 measurement to investigate extracellular matrix turnover in bovine models of mastitis, laminitis, postpartum uterine involution, and osteoarthritis, where collagen degradation products serve as biomarkers of tissue remodeling and inflammatory progression. Each kit contains a pre-coated microplate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, and chromogenic substrate reagents, and the protocol is optimized for serum-based, plasma-based, and tissue homogenate sample matrices. The procedure typically involves a 30 minute incubation at 37 °C for sample binding and a 15 minute chromogenic development step before absorbance is recorded. Store all unopened components refrigerated; allow reagents to equilibrate to room temperature before running the assay to ensure reproducible standard curves. Aliquot standards to limit freeze-thaw cycles and protect the substrate from prolonged light exposure. The kit is intended for research use only and is not validated for diagnostic procedures.