Bovine ITGAL (Integrin, alpha L) ELISA Kit (BOFI00137)

ITGAL (Integrin alpha L), also designated CD11a, pairs with the beta-2 integrin CD18 to form Lymphocyte Function-Associated Antigen-1 (LFA-1), a heterodimeric adhesion molecule central to leukocyte trafficking, immune synapse formation, and T-cell co-stimulation in cattle. This Assay Genie kit is configured for research use only and supports quantitative measurement of Integrin alpha L in bovine specimens through an absorbance-based immunoassay workflow run on a standard microplate reader, with reagents supplied for 96 Assays per kit.

The assay is intended for in vitro studies of bovine immune function, including investigations into neutrophil and lymphocyte adhesion, inflammatory responses to pathogens such as bovine respiratory disease complex, mastitis-associated leukocyte infiltration, and vaccine-elicited cell-mediated immunity. Researchers studying CD11a/LFA-1 expression on peripheral blood mononuclear cells, lymph node tissue, or splenocytes from cattle can apply the kit to blood-derived fractions collected under established veterinary sampling protocols, helping to clarify the contribution of the alpha-L subunit to cell-cell interactions in species-specific infection models.

Reagents are provided in individual vials and should be stored according to the package insert to preserve antigen-binding activity across the run. The kit is supplied in a pack of one format, with all buffers, reference standards, and labelled detection antibody components prepared for immediate use in the laboratory workflow.