Bovine Interleukin 18 (IL-18) ELISA Kit

The Bovine Interleukin 18 (IL-18) ELISA Kit from Assay Genie quantifies the pro-inflammatory cytokine IL-18 in bovine serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants. IL-18, originally identified as interferon-gamma-inducing factor, plays a central role in innate and adaptive immunity, driving IFN-gamma production, NK cell activation, and Th1 differentiation.

Key specifications include a detection range of 31.25 to 2000 pg/mL, an assay sensitivity below 9.375 pg/mL, and a sample volume of 100 µL per well. The microplate is precoated with a bovine-specific capture antibody and paired with a biotinylated detection reagent plus HRP-streptavidin conjugate, producing an enzyme-mediated signal readable on a standard plate reader. Unopened kit components should be kept refrigerated; reconstituted standards must be aliquoted and frozen at -20°C to limit freeze-thaw cycles.

Veterinary and biomedical researchers apply this assay to characterize inflammatory responses, evaluate vaccine efficacy, monitor infectious disease models, and study reproductive and metabolic disorders in cattle, where circulating IL-18 levels serve as a biomarker of innate immune activation.