Bovine Insulin-like growth factor-binding protein 6 (IGFBP6) ELISA Kit

IGFBP6 is a key regulator of insulin-like growth factor (IGF) signaling, predominantly binding IGF-2 with high affinity and modulating cellular proliferation, differentiation, and apoptosis. From Assay Genie, this Sandwich ELISA Kit is built around a 96-well microplate pre-coated with a capture antibody specific to bovine IGFBP6, paired with a biotinylated detection antibody and a streptavidin-HRP conjugate for colorimetric readout. The assay is calibrated for accurate quantitative measurement of IGFBP6 in bovine serum and plasma samples, with minimal cross-reactivity to related IGF binding proteins.

During the workflow, standards and samples are added to the wells, followed by sequential incubation and wash steps; the chromogenic substrate is then developed, the reaction is stopped, and absorbance is read at 450 nm on a standard microplate reader. For reliable results, store all unopened components at 2-8°C, protect the substrate from light, and avoid repeated freeze-thaw cycles of the reconstituted standard. The kit is intended for research use only and is compatible with downstream quantitative ELISA analysis in cell culture supernatants, tissue homogenates, and other biological fluids.