Bovine Insulin Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit

A quantitative immunoassay from Assay Genie for measuring Insulin-like Growth Factor Binding Protein 4 (IGFBP4) in bovine samples, supplied as a complete kit with a pre-coated microplate, assay diluent, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and TMB substrate.

IGFBP4 is a 28 kDa secreted glycoprotein that binds IGF-1 and IGF-2 with high affinity, modulating local insulin-like growth factor signaling in reproductive, mammary, and hepatic tissues. The kit is used in studies of bovine ovarian follicular development, corpus luteum regression, lactation biology, and somatotropic-axis physiology, with applications including screening of cell culture supernatant and tissue homogenate samples.

The protocol follows a standard quantitative ELISA workflow, with chromogenic signal read on a microplate reader after stopping the reaction; calibration curves are fitted using a four-parameter logistic model across the supplied standards. For research use only; not intended for diagnostic, therapeutic, or veterinary clinical applications.

Reagents ship with cold packs; unopened kits should be stored refrigerated and protected from light to maintain stability through the stated shelf life. Each kit contains materials sufficient for one microplate run with multiple sample replicates.