Bovine Inhibitor of nuclear factor kappa-B kinase subunit beta (IKBKB) ELISA Kit

This Sandwich ELISA Kit from Assay Genie quantifies IKBKB in bovine serum and plasma samples using a colorimetric detection method. IKBKB (also known as IKK-β or IKK2) is a serine/threonine kinase central to NF-κB signal transduction, IκBα phosphorylation, and downstream inflammatory gene expression. Researchers investigating bovine immunology, mastitis, metabolic stress, and inflammation models use this assay to measure endogenous IKBKB protein in cell culture supernatants and bodily fluids.

The kit is supplied in a 96-well microplate format with pre-coated capture antibodies, lyophilized standards, detection reagents, and a complete protocol. The assay typically requires 50 µl of sample per well, a short incubation, and absorbance read at 450 nm with a standard microplate reader. Standard curves are generated from a serial dilution series. Store unopened components refrigerated at 2-8 °C; reconstituted reagents should be aliquoted and frozen at -20 °C to limit freeze-thaw cycles and preserve activity through the stated shelf life.

For research use only. Not for diagnostic or therapeutic procedures.