Bovine IL5 (Interleukin-5) ELISA Kit (BOFI00115)

The Assay Genie bovine interleukin-5 kit provides quantitative measurement of IL-5 in cell-free bovine samples, employing a paired-antibody capture approach on a pre-coated microplate. The assay uses matched capture and detection antibodies, recombinant standards, and substrate reagents sufficient for a full plate run, with absorbance measured after the enzymatic reaction. Typical intra-assay precision is below 10% CV, and the reagents show no significant cross-reactivity with related bovine cytokines such as IL-3, IL-4, or GM-CSF. Researchers commonly apply this kit to study eosinophil biology, Th2 immune responses, and parasitic infection models in cattle. The standard curve range spans 15.6 to 1000 pg/mL with a sensitivity of approximately 5 pg/mL, and the protocol uses 100 uL of sample per well with a total incubation time of around 3 hours. All components should be kept refrigerated upon arrival and protected from light, with vials returned to cold storage between uses to preserve reagent activity.