Bovine IL13 (Interleukin-13) ELISA Kit (BOFI00114)

According to the Assay Genie validation data, this double-antibody ELISA uses biotinylated detection antibody plus HRP-Streptavidin (SABC) signal amplification, with the full detection protocol completing in approximately 4 hours excluding plate equilibration and sample preparation, run at 37 °C incubation temperatures. Intra-assay CV is consistently below 6% (typically 4.8-5.3%) and inter-assay CV is below 6%, with spike-recovery of 86-102% in serum, 88-103% in EDTA plasma, and 89-101% in heparin plasma, and parallel dilution linearity of 85-105% across the same matrices. The corresponding catalogue reference number is EB0171. Beyond serum or plasma, the kit has been verified on cell culture supernatant, cell or tissue lysates, and other liquid samples, broadening its usefulness for in vitro work on isolated bovine leukocytes. Sealed kit components remain stable for 12 months under refrigerated storage, with accelerated stability testing showing approximately 80% retained activity after 1 month at 37 °C and 95-100% retained activity through 6 months refrigerated. Researchers studying gastrointestinal nematode resistance (for example Cooperia oncophora), Mannheimia haemolytica and BRSV-associated bovine respiratory disease, subclinical and clinical mastitis, and Neospora caninum pregnancy infection have all relied on circulating or local IL-13 measurements in cattle to characterise Th2 versus Th1 polarisation, making this Assay Genie kit a practical tool for herd-health surveillance, vaccine efficacy trials, and immunophenotyping of mucosal or systemic responses.