Bovine IL-4 (Interleukin 4) ELISA Kit (BOFI00130)

From Assay Genie, this double-antibody sandwich ELISA kit is designed for the accurate quantification of bovine Interleukin 4 (IL-4) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. IL-4 is a key cytokine that regulates immune response and inflammation and is implicated in allergic reactions, autoimmune diseases, and host defense mechanisms, supporting research into bovine health and disease pathogenesis.

The colorimetric assay is read at 450 nm and delivers a sensitivity of 9.375 pg/mL with a working standard curve of 15.625-1000 pg/mL. Validated linearity spans 80-105% across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, while spike-and-recovery averages 95-96% across the same matrices. Unopened reagents are stored at 2-8°C for 12 months.

The workflow combines reagent equilibration, primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody binding for 60 minutes, HRP-Streptavidin (SABC) incubation for 30 minutes, TMB color development in the dark for 10-20 minutes, and absorbance reading at 450 nm after stop solution. Provided sample-preparation guidance covers serum clotting, plasma anticoagulant collection, tissue homogenisation in PBS with protease inhibitors, cell culture supernatant clarification, and cell lysis. The kit is also known by the synonyms IL-4, IL4, BCGF-1, BCGF1, BSF-1, and BSF1.