Bovine IL-21 DIY ELISA Kit

Interleukin-21 (IL-21) is a pleiotropic type I cytokine central to B cell maturation, T follicular helper cell development, and NK cell cytotoxic activity, and quantifying its concentration in bovine samples is a routine need in veterinary immunology research. This Assay Genie kit addresses that need through a flexible do-it-yourself plate-coating format that delivers the capture antibody, biotinylated detection antibody, recombinant bovine IL-21 standard, streptavidin-HRP conjugate, and assay buffers required to assemble an in vitro immunoassay in the user's own laboratory.

Sized to support up to 10 plates, the workflow scales readily across longitudinal vaccination studies, dose-response experiments, or herd-level comparisons. Researchers investigating bovine respiratory syncytial virus, Mycobacterium avium subspecies paratuberculosis, Staphylococcus aureus mastitis, or bovine tuberculosis have applied IL-21 quantification to peripheral blood mononuclear cell cultures stimulated with recall antigens, supporting evaluation of cell-mediated immune responses in natural host-pathogen settings.

Reagents ship with cold-chain handling and should be stored refrigerated between 2 to 8 degrees C upon arrival. Reconstituted working solutions typically remain active for short-term use under refrigerated conditions; refer to the product insert for component-specific stability, aliquoting guidance, and shelf-life details. Each kit is supplied as a single unit configured for research use only and is not validated for diagnostic or clinical procedures.