Bovine IL-17A (Interleukin-17) ELISA Kit (BOFI00124)

This quantitative cytokine immunoassay from Assay Genie measures IL-17A levels in bovine specimens, supporting studies of inflammatory signaling, host-pathogen responses, and vaccine adjuvancy in cattle. The kit includes a pre-coated microplate with strip wells, lyophilized recombinant standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash concentrate, TMB substrate, and stop solution. Standard curve range is 15.6 to 1000 pg/mL with sensitivity below 10 pg/mL, allowing resolution of low-abundance cytokine shifts. Protocols call for 100 uL of diluted sample loaded per well in duplicate, with absorbance read after chromogenic development. Unopened components should be stored refrigerated and protected from light; reconstituted standards remain stable for short-term use and working dilutions prepared fresh per run. Antibodies are bovine-specific with no significant cross-reactivity reported against related cytokines such as IL-17F. For research use only; not approved for diagnostic or clinical procedures.