Bovine IL-13 DIY ELISA Kit

The Assay Genie Bovine IL-13 DIY ELISA Kit is a flexible, do-it-yourself immunoassay supplied in bulk components to coat up to 10 microplates, allowing researchers to configure antibody pairing and plate layout for their own workflows. IL-13 is a Th2-associated cytokine implicated in parasitic infection, allergic inflammation, and mucosal immunity in cattle, making this kit useful for vaccine response studies, respiratory disease research, and mastitis investigations in dairy herds.

Capture and detection antibodies, recombinant bovine IL-13 standard, and streptavidin-HRP reagent are provided concentrated for refrigerated storage. The standard curve yields a detection range of 31.25 to 2000 pg/mL with sensitivity under 10 pg/mL, and the protocol requires a 100 µL sample volume per well. Researchers coat their own plates, then add serum or plasma samples, or cell culture supernatant, alongside the recombinant IL-13 standard.

Substrate is read on a standard microplate reader, and the DIY format makes this kit an economical option for laboratories running multiple cytokine panels or longitudinal bovine immunity studies. The kit is intended for research use only.