Bovine IL-1 alpha DIY ELISA Kit

Pro-inflammatory cytokine IL-1 alpha is central to immune activation in cattle, with documented involvement in mastitis, respiratory infections, and inflammatory cascades. This Assay Genie reagent set provides capture antibody, biotinylated detection antibody, recombinant standard, streptavidin-HRP, coating buffer, wash buffer, and substrate solution, giving laboratories flexibility to coat and run up to ten microplates from a single kit. The DIY format fits projects where off-the-shelf precoated kits do not match throughput, and standard curves can be run in duplicate or triplicate depending on plate count.

Common research applications include measuring cytokine release in cell culture supernatant from stimulated bovine leukocytes, quantifying inflammatory mediators in mastitic milk, and tracking IL-1 alpha concentrations in serum samples collected during vaccination or challenge studies. Components are shipped refrigerated; reconstituted standard should be frozen in aliquots to limit freeze-thaw degradation. For research use only.