Bovine IFN-alpha PharmaGenie ELISA Kit (SBRS0187)

From the Assay Genie PharmaGenie reagent family, this immunoassay targets bovine interferon-alpha (IFN-α), a type I cytokine central to antiviral defense, innate immune signaling, and the regulation of cell-mediated immunity in cattle. Quantifying IFN-α in experimental samples supports veterinary immunology research, vaccine efficacy studies, and investigations of bovine respiratory disease and viral infection models.

The reagents are configured for 96 determinations and rely on antibody pairs directed against bovine IFN-α, with binding revealed through an enzyme-conjugated reporter system that produces a measurable color change in each well of the supplied microplate. The kit provides pre-coated wells, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant bovine IFN-α standard, wash buffer, substrate solution, and stop solution. A typical run uses a 100 µL sample volume per well, with standards assayed in duplicate to build a calibration curve and absorbance read on a standard microplate reader.

Unopened reagents should be stored refrigerated at 4 °C according to the package insert; reconstituted standards and working dilutions are best aliquoted and frozen to limit freeze-thaw cycles and preserve long-term activity.