Bovine IFI30 (Gamma-interferon-inducible lysosomal thiol reductase) ELISA Kit (BOFI00133)

The Assay Genie bovine IFI30 immunoassay targets GILT, an interferon-gamma-inducible lysosomal thiol reductase that catalyzes disulfide bond reduction in proteins destined for MHC class II antigen presentation. This plate-based quantitative kit supports immunology, infectious disease, and vaccine research in cattle, including studies of bovine tuberculosis, paratuberculosis, and mycobacterial immune responses. The procedure uses pre-coated microplate wells, ready-to-use reagents, and a lyophilized standard for curve generation. Species-specific capture and detection antibodies minimize cross-reactivity with non-bovine homologs, ensuring accurate quantification in cattle-derived specimens. Each kit contains a sealed microplate, diluent buffers, detection antibody, and substrate vial; refrigerate all components at 4 °C and protect from light to maintain enzymatic activity. Avoid repeated freeze-thaw cycles of the lyophilized standard by preparing single-use aliquots immediately after reconstitution.