Bovine HSP90 (Heat shock protein 90) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the accurate quantification of HSP90 (Heat Shock Protein 90) levels in bovine samples, including serum, plasma, and cell culture supernatants. The kit uses a double-antibody sandwich immunoassay with colorimetric detection via TMB substrate, with absorbance measured at 450 nm. It reports a sensitivity of 3.75 ng/mL and a working range of 6.25–400 ng/mL, supporting research into HSP90, a chaperone protein central to protein folding, stability, and cellular stress responses, and implicated in conditions including cancer, neurodegenerative disorders, and inflammation.

Validated performance has been demonstrated in serum, EDTA plasma, and heparin plasma matrices. Linearity across 1:2, 1:4, and 1:8 dilutions spans 85–104% for serum, 83–101% for EDTA plasma, and 81–100% for heparin plasma. Recovery ranges are 86–100% (average 93%) for serum, 88–105% (average 96%) for EDTA plasma, and 89–103% (average 96%) for heparin plasma. The kit is supplied with components including biotin-labeled detection antibody, HRP-streptavidin conjugate, TMB substrate, and assay buffers, and should be stored at 2–8°C for up to 12 months.