Bovine HSP70 (Heat shock protein 70) ELISA Kit

Designed for the accurate quantification of HSP70 in bovine research samples, this Sandwich ELISA from Assay Genie uses a colorimetric detection method. The kit is reactive with bovine samples and has been validated for use with serum, plasma, cell culture supernatant, cell lysis buffer or tissue lysis buffer, and other biological fluid samples.

Reported performance includes a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL. Linearity in serum (n=5) is reported at 85-101% (1:2), 85-99% (1:4), and 92-102% (1:8); in EDTA Plasma (n=5) 86-104%, 86-101%, and 82-100%; in Heparin Plasma (n=5) 88-101%, 84-100%, and 81-100%. Recovery is reported as 85-104% (average 93%) for serum (n=5), 88-104% (average 99%) for EDTA Plasma (n=5), and 97-104% (average 99%) for Heparin Plasma (n=5). HSP70 is described as a key protein involved in the cellular stress response, with roles in protecting cells from damage and maintaining protein homeostasis, and with implications in research on heat shock, inflammation, and neurodegenerative diseases. The kit is stored at 2-8°C for 12 months.