Bovine Heat Shock Protein 90kDa Alpha A1 (HSP90aA1) ELISA Kit

The Assay Genie Bovine HSP90aA1 ELISA Kit is a quantitative immunoassay for measuring the inducible 90 kDa heat shock protein alpha A1 in bovine samples. HSP90aA1 functions as an ATP-dependent molecular chaperone involved in protein folding, signal transduction, and the cellular stress response, making it a relevant biomarker for bovine physiology, veterinary research, and livestock studies.

Supplied as a complete kit with reagents sufficient for 96 determinations, the set includes a pre-coated microplate, biotinylated detection antibody, recombinant standards, and chromogenic substrate. The protocol uses a 50 uL sample volume and the capture antibody selectively recognizes bovine HSP90aA1 with no significant cross-reactivity with rat or human HSP90aA1 homologs, supporting accurate quantification of the bovine target.

Typical applications include heat shock response studies, chaperone expression profiling, and stress biomarker work in serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants. Store reagents at the recommended temperature and avoid repeated freeze-thaw cycles of working solutions to preserve assay performance.